EPAS1 Knockout cell line (Hep G2)

×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location: Home > Products > KO Cell Lines > EPAS1 Knockout cell line (Hep G2)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • CRISPR-iScreen™ Library Plasmid
  • CRISPR-iScreen™ Library Virus
  • CRISPR-iScreen™ Cell Pool
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

EPAS1 Knockout cell line (Hep G2) Order

EPAS1 Knockout cell line (Hep G2)
Catalog#
YKO-HT1984
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
EPAS1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
EPAS1 Knockout cell line (Hep G2)
Species
Human
Gene Name
EPAS1
Gene ID
2034
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript EPAS1-201)
Validation: PCR+Sanger Sequencing
EPAS1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the EPAS1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, EPAS1 gene knockout EPAS1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
EPAS1 Knockout cell line (Hep G2)
EPAS1 Knockout cell line (Hep G2)
Catalog#
YKO-HT1984
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • EPAS1 Knockout cell line (HeLa)

    EPAS1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: EPAS1
    Gene ID: 2034
    Size: 1*10^6
    Catalog#: YKO-XN24816
    YKO-XN24816(HeLa)
  • EPAS1 Knockout cell line (HCT 116)

    EPAS1 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: EPAS1
    Gene ID: 2034
    Size: 1*10^6
    Catalog#: YKO-KP235
    YKO-KP235(HCT 116)
  • EPAS1 Knockout cell line (HEK293)

    EPAS1 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: EPAS1
    Gene ID: 2034
    Size: 1*10^6
    Catalog#: YKO-XN24814
    YKO-XN24814(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • EPAS1 Knockout cell line (HeLa)

    EPAS1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: EPAS1
    Gene ID: 2034
    Size: 1*10^6
    Catalog#: YKO-XN24816
  • EPAS1 Knockout cell line (HCT 116)

    EPAS1 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: EPAS1
    Gene ID: 2034
    Size: 1*10^6
    Catalog#: YKO-KP235
  • EPAS1 Knockout cell line (HEK293)

    EPAS1 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: EPAS1
    Gene ID: 2034
    Size: 1*10^6
    Catalog#: YKO-XN24814

Contact us